Recent Publications From The Lab

Cai, Y. and J. G. Mikkelsen. 2014. Driving DNA transposition by lentiviral protein transduction. Mobile Genetic Elements, 4:e29591.

 

Cai, Y., R. O. Bak, and J. G. Mikkelsen. 2014. Targeted genome editing by lentiviral protein transduction of zinc-finger and TAL-effector nucleases. eLife, 3:e01911.

 

Bjørklund, M. M., A. K. Hollensen, M. K. Hagensen, F. Dagnæs-Hansen, C. Christoffersen, J. G. Mikkelsen, and J. F. Bentzon. 2014. Induction of atherosclerosis in mice and hamsters without germline genetic engineering. Circulation Research, 114:1684-1689.

 

Cai, Y., R. O. Bak, L. B. Krogh, N. H. Staunstrup, B. Moldt, T. J. Corydon, L. D. Schrøder, and J. G. Mikkelsen. 2014. DNA transposition by protein transduction of the piggyBac transposase from lentiviral Gag precursors. Nucleic Acids Research 42(4):e28.

 

Skipper, K. A., P. R. Andersen, N. Sharma, and J. G. Mikkelsen. 2013. DNA transposon-based gene vehicles – scenes from an evolutionary drive. J. Biomedical Science 20:92. 


Bak, R. O., A. K. Hollensen, and J. G. Mikkelsen. 2013. Managing microRNAs with vector-encoded decoy-type inhibitors. Molecular Therapy, advanced online publication.

 

Staunstrup, N. H., R. O. Bak, Y. Cai, L. Svensson, T. K. Petersen, C. Rosada, K. Stenderup, L. Bolund, J. G. Mikkelsen. 2013. A lentiviral vector-based genetic sensor system for comparative analysis of permeability and activity of vitamin D3 analogues in xenotransplanted human skin. Experimental Dermatology, 22:178-183.

 

Sharma, N., Y. Cai, R. O. Bak, M. R. Jakobsen, L. D. Schrøder, and J. G. Mikkelsen. 2013. Efficient Sleeping Beauty DNA transposition from DNA minicircles. Molecular Therapy - Nucleic Acids, 2:e74.

 

Hollensen, A. K., R. O. Bak, D. Haslund, and J. G. Mikkelsen. 2013. Suppression of microRNAs by dual-targeting and clustered Tough Decoy inhibitors. RNA Biology, 10(3) Epub ahead of print.

 

Bak, R. O., A. K. Hollensen, M. N. Primo, C. D. Sørensen., and J. G. Mikkelsen. 2013. Potent miRNA suppression by RNA Pol II-transcribed Tough Decoy inhibitors. RNA, 19:280-293.

 

Al-Mashhadi, R. H., C. B. Sørensen, P. M. Kragh, C. Christoffersen, M. B. Mortensen, L. P. Tolbod, T. Thim, Y. Du, J. Li, Y. Liu, B. Moldt, M. Schmidt, G. Vajta, T. Larsen, S. Purup, L. Bolund, L. B. Nielsen, H. Callesen, E. Falk, J. G. Mikkelsen, and J. F. Bentzon. 2013. Familial hypercholesterolemia and atherosclerosis in cloned minipigs created by DNA transposition of a human PCSK9 gain-of-function mutant. Science Translational Medicine, 5(166):166ra1.

 

Sharma, N., A. K. Hollensen, R. O. Bak, N. H. Staunstrup, L. D. Schrøder, and J. G. Mikkelsen. 2012. The impact of cHS4 insulators on DNA transposon vector mobilization and silencing in retinal pigment epithelium cells. PLoS One, 7(10):e48421.

Primo, M. N, R. O. Bak, B. Schibler, and J. G. Mikkelsen. 2012.  Regulation of pro-inflammatory cytokines TNFalpha and IL24 by microRNA-203 in primary keratinocytes. Cytokine, 60:741-748.

 

Staunstrup, N. H., J. Madsen, M. N. Primo, J. Li, Y. Liu, P. M. Kragh, R. Li, M. Schmidt, S. Purup, F. Dagnæs-Hansen, L. Svensson, T. K. Petersen, H. Callesen, L. Bolund, and J. G. Mikkelsen. 2012. Development of transgenic cloned pig models of skin inflammation by DNA transposon-directed ectopic expression of human beta1 and alpha2 integrin. PLoS One 7(5):e36658.

Primo, M. N., R. O. Bak, and J. G. Mikkelsen. 2012. Lentiviral vectors for cutaneous RNA managing. Experimental Dermatology, 21:162-70.

 

Staunstrup, N. H. and J. G. Mikkelsen. 2011. Integrase-defective lentiviral vectors – a stage for nonviral integration machineries. Current Gene Therapy, 11:350-362.

Bak, R. O. and J. G. Mikkelsen. 2011. Mobilization of DNA transposable elements from lentiviral vectors. Mobile Genetic Elements, 1:139-144

 

Staunstrup, N. H., N. Sharma, R. O. Bak, L. Svenson, T. K. Petersen, L. Aarenstrup, K. Kristiansen, L. Bolund, and J. G. Mikkelsen. 2011. A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues. BMC Biotechnology, 11:33.

 

Moldt, B., C. Miskey, N. H. Staunstrup, A. Gogol-Döring, R. O. Bak, N. Sharma, L. Mates, Z. Izsvak, W. Chen, Z. Ivics, and J. G. Mikkelsen. 2011. Comparative genomic integration profiling of Sleeping Beauty transposons mobilized with high efficacy from integrase-defective lentiviral vectors in primary human cells. Molecular Therapy, Epub ahead of print.

 

Bak, R. O., K. Stenderup, C. Rosada, L. B. Petersen, B. Moldt, F. Dagnæs-Hansen, M. Jakobsen, S. Kamp, T. G. Jensen, T. N. Dam, and J. G. Mikkelsen. 2011. Targeting of human interleukin-12B by monoclonal antibodies and small hairpin RNAs in xenografted psoriatic skin. BMC Dermatology, 11:5.

 

Jakobsen, J. E., P. M. Kragh, B. Moldt, J. Li, M. Schmidt, B. D. Schyth, N. Lorenzen, G. Vajta, L. Bolund, H. Callesen, A. L. Jørgensen, A. L. Nielsen, and J. G. Mikkelsen. 2010. Pig transgenesis by Sleeping Beauty DNA transposition. Transgenic Research, Aug. 2010, Epub ahead of publication.

Bak, R. O and J. G. Mikkelsen. 2010. Regulation of cytokines by small RNAs during skin inflammation. Journal of Biomedical Science 17:53 (1-19).

 

Jakobsen, M., K. Stenderup, C. Rosada, B. Moldt, S. Kamp, T. N. Dam, T. G. Jensen, and J. G. Mikkelsen. 2009. Amelioration of psoriasis by anti-TNFalpha RNAi in the xenograft transplantation model. Mol. Ther. 17:1743-1753.

 

Staunstrup, N. H., B. Moldt, L. Mates, P. Villesen, M. Jakobsen, Z. Ivics, Z. Izsvak, and J. G. Mikkelsen. 2009. Hybrid lentivirus-transposon vectors with a random integration profile in human cells. Mol. Ther., 17:1205-1214.

 

Dalsgaard, T., B. Moldt, N. Sharma, G. Wolf, A. Schmitz, F. S. Pedersen, and J. G. Mikkelsen. 2009. Shielding of Sleeping Beauty DNA transposon-based transgene cassettes by heterologous promoters in early embryonal cells. Mol. Ther. 17:121-130.

 

Sharma, N. B. Moldt, T. Dalsgaard, T. G. Jensen, and J. G. Mikkelsen. 2008. Regulated gene insertion by steroid-induced ФC31 integrase. Nucleic Acids Res 36:e67.

 

Moldt, B., N. H. Staunstrup, M. Jakobsen, R. J. Yanez-Munoz, and J. G. Mikkelsen. 2008. Genomic insertion of lentiviral DNA circles directed by the yeast Flp recombinase. BMC Biotechnology 8(1):60.

 

Moldt, B., S. R. Yant, P. R. Andersen, M. A. Kay, and J. G. Mikkelsen. 2007. Cis-acting regulatory activities in the terminal regions of Sleeping Beauty DNA transposon-based vectors. Human Gene Therapy, 18:1193-1204.